Product Information

histologyPotApproved

Solvents

IDA 99% (+ Various Concentrations)
Denatured alcohol, also called methylated spirits, industrial methylated spirits, is ethanol that has additives to make it poisonous. Used for the removal of aqueous solutions during tissue processing and staining.

Xylene
Clearing agent used in tissue processing and during the staining process.

Acquaclear
An alternative clearing agent.

Methanol
Used as a fixative for blood cell in Haematogy.

Acetone
For hardening and dehydrating tissues, and is often used as a decolourising agent in some staining protocols.

Isopropanol (IPA)
Common laboratory solvent

Acid Alcohol 0.5%
Differentiation solution for removing excess haematoxylin from tissues to accentuate structure and cell clarification

Acid Alcohol 1%
Differentiation solution for removing excess haematoxylin from tissues to accentuate structure and cell clarification

Fixatives

Neutral Buffered Formalin 10%
Ready to use general purpose specimen fixative.

Neutral Buffered Formalin Concentrate
Concentrated buffered formalin solution for specimen fixation.

Formal Saline 10%
Ready to use solution of formal saline.

Formal Saline Concentrate
Concentrated solution of formal saline.

Davidson’s Solution
Davidson solution is a rapid fixative. It is particularly useful for preparing tumors, bone marrow specimens, gynecologic material, fatty breast, and medical biopsies. It may be used for testicular biopsies. In addition, Davidson Solution is the preferred fixative for whole eyes, which provides optimal tissue preservation while avoiding retinal detachment.

Bouins Fixative
Excellent for use in preserving soft and delicate tissue structures.

Stains

Haematoxylin

Harris (Acidified)
An alum haematoxylin, which uses chemical ripening to produce oxidation of haematoxylin for immediate use. It is one of the most common routine haematoxylin used in today’s modern histology laboratory. Ripened with acetic acid for enhanced nuclear staining.

Gill’s I
Lower strength formulation, ideal for staining cytology.

Gill’s II
Intermediate formulation, used as a counterstain for immunohistochemistry (IHC) and for more intense cytological staining.

Gill’s III
Strongest strength formulation for histological staining of nuclei with reduced staining times.

Mayer’s
A more powerful stain than Ehrlich’s haematoxylin and a precise nuclear stain which is used progressively, although, it may be used regressively with a staining time of 5-10 minutes.

Weigert’s Iron Solution A
Used for the staining of cell nuclei when subsequent staining reagents contain acid such as in van Gieson stain which will decolourise nuclear staining if stained previously with a solution of haematoxylin which contains potassium alum or ammonium alum as the mordant. The Weigert’s haematoxylin, which is 1% alcoholic haematoxylin, is stored separately from the mordant, which is acidified ferric chloride. Equal volumes are mixed immediately before use. The resulting colour should be purplish black with a staining time of 20-30 minutes. Weigert’s iron haematoxylin is used for the staining of cell nuclei when demonstrating collagen and muscle with the van Gieson stain and the trichrome connective tissue stains.

Weigert’s Iron Solution B
Solution B is the acidified ferric chloride, used in combination with Solution A

Heidenhain’s
A regressive cytological stain which stains tissue jet black, and by careful selective differentiation, many tissue and cell components can be revealed in shades of black and grey. This makes it useful for photomicrography. Being a cytological stain, tissue sections must be very thin to enable easy demonstration of cell constituents. Heidenhain’s iron haematoxylin will demonstrate mitochondria, chromatin, chromosomes, nucleoli, centrioles, nuclear membrane, cross-striations of muscle fibres and myelin. Red blood cells are stained black.

Eosin

Eosin (1 % Alcoholic)

Alcoholic Eosin solution. Eosin Y is the most common counterstain in the Haematoxylin and Eosin method (H&E) used as a cytoplasmic stain to demonstrate general architecture of a tissue.

Eosin (1% Aqueous)
Aqueous Eosin solution. Eosin Y is the most common counterstain in the Haematoxylin and Eosin method (H&E) used as a cytoplasmic stain to demonstrate general architecture of a tissue.

Mounting Medium

DPX Mountant
Histology mountant consisting of a mixture of distyrene, a plasticizer, and xylene used as a synthetic resin mounting media. Dries quickly, preserves stain and can be customised to suit your choice of automated coverslipper.

Further special staining kits and stains for Histology, Cytology and Haematology are available on request.